What are you looking for?

MALDI-TOF-MS Automated Sample Preparation for uHTS

MALDI-TOF-MS Sample Preparation

Bringing the analytical power of MALDI-TOF-MS to uHTS

  • MALDI spotting of 1536 samples in <10 minutes
  • Parallel transfers of 96, 384 and 1536 samples with CyBio Well vario
  • No interferences thanks to intelligent washing routines
  • Highly homogenous target spots thanks to active sample drying
  • Highly precise spotting for reliable, automated analysis
  • Software for process planning, matrix injection, washing and drying

Several hundred thousand samples in a single run

Analytik Jena brings extensive automation and liquid handling experience to automated sample preparation for uHTS applications supplying a walk away automation solution for MALDI-TOF-MS able to handle several hundred thousand samples in a single run.

An ultra-high-throughput automation solution

With matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS) mass spectrometry is now available for high throughput screening (HTS) e.g., in drug discovery. The Bruker Rapiflex MALDI-TOF-MS is rapidly becoming the instrument of choice for HTS and ultra HTS (uHTS) in pharma, academia and the commercially focused contract environment. It is the fastest mass spectrometer on the market enabling collection of real-time data in high throughput high density when automated into a robotic sample preparation system. Analytik Jena supplies all application demands for a reliable MALDI automation, from a simple workstation to a complex integration. The solution enables 1536 samples to be spotted onto a MALDI target plate in less than 10 minutes facilitating continuous sample reads in a screening environment.

Small assay volume and homogenous spot generation for MALDI

The liquid handling demands for sample preparation in high-density are diverse. The robust and precise CyBio Well vario 1536 pipettor ensures sample integrity and result reproducibility in either 384‑ or 1536-well formats by spotting an entire target plate in a single transfer.

Learn more

MALDI sample clean up enables the reduction of salt interferences

On-target washing enhances detection capabilities of target molecules in complex assay buffers and thus accelerates assay development. The desalting function of on-target-washing significantly improves both the assay conditions and the measurement results. The CyBio Well vario Head 1536/8μl can complete on target-washing for 1536 samples in less than 60 s.

Active Drying provides extremely homogenous spot shapes

The "Active Dryer" is an important element of the automation for MALDI-TOF-MS sample preparation. By enabling controlled crystallization conditions, the active dryer allows faster sample preparation and creation of extremely homogeneous sample spots.

Smart accessories perfectly supply the needs of MALDI sample preparation

To avoid sample carry-over, tip washing between spotting of assays plates is essential. The CyBio Well vario offers automated and optimized tip washing steps in 384- or 1536-well format. Thanks to highly efficient washing stations, no cross-contaminations can be detected following only three washing cycles.

Modular and customized automation solutions

Based on a modular system, the degree of automation can be freely configured by the customer. In addition to high-quality liquid handling equipment, Analytik Jena also offers fully automated automation solutions tailored to the respective customer needs.

Learn more

Software

Software is the key element for automation systems. Our powerful and scalable software packages allow detailed control of all components for MALDI-TOF sample preparation as well as easy handling of the complex scheduling tasks.Thanks to its sophisticated software and scheduling concept the HTS CyBio automation solution for MALDI-TOF-MS sample preparation is capable of preparing the specific sample plates within the same time as the mass spectrometer can measure them (< 10 min). This allows the screening of one million samples in less than five days.

  • CyBio Composer
  • CyBio Scheduler
  • CyBio AppStudio

Air-Drying Versus Active Drying

Active sample drying has a significant impact on the quality of subsequent analysis. The following comparison images illustrate how you can generate more homogeneous and thus higher-quality spots by actively drying MALDI-TOF-MS samples.

Air Drying: DHBA in 50 % Acetonitril +0,1 % TFA

Active Vacuum Drying: DHBA in 50 % Acetonitril +0,1 % TFA

Air Drying: Sinapinic Acid in 75% Acetonitril + 0,1% TFA

Active Vacuum Drying: Sinapinic Acid in 75% Acetonitril + 0,1% TFA

Air Drying: HCCA in 70 % Methanol + 0,1 % TFA

Active Vacuum Drying: HCCA in 70 % Methanol + 0,1 % TFA

  • DHBA air dried1
  • DHBA actively dried2
  • Sinapinic acid air dried3
  • Sinapinic acid actively dried4
  • HCCA air dried5
  • HCCA actively dried6

Technology MALDI Sample Preparation

Learn more

Configuration Examples MALDI Sample Preparation

Learn more

Automation Systems for MALDI-TOF-MS Sample Preparation

MALDI-TOF Sample Prep – Scale up to 1 Mio. Samples a Week

Watch now

Web Seminars

Request consultation

The newsletter of Analytik Jena frequently keeps you posted about:

  • News
  • Trends and developments
  • Events

Sign up here

This website uses cookies

To optimize our website and offer you relevant content and personalized advertising, we access information (e.g. device recognition, browser information) in your terminal device and store this information (such as cookies or other technologies) in your terminal device. In this way, we measure how and with what you use our offers. We also share the (personal) data collected in the process with third parties. By clicking “Accept all”, you agree (revocable at any time for the future) to the storage and data processing.

In the settings you can individually adjust your voluntary consent at any time or grant/withdraw consent for individual purposes. You can find more information in the privacy policy.

If you are under 16 and wish to give your consent to voluntary services, you must ask your legal guardian for permission.

Note on data transfer outside the EU: In some cases, data might be processed outside the European Union in the context of the use of third-party services. This only takes place if the special requirements of Art. 44 et seq. GDPR are fulfilled. Data is transmitted for the purpose of compiling statistics and implementing marketing measures. Further information can be found in our privacy policy.

Consent Management

We respect your right to privacy. You can decide for yourself which types of cookies you want to accept when visiting the website and which not.

Category Description Status
Necessary These cookies are responsible for the provision of the most important website functionalities, e.g. for saving data protection and content settings. They also enable us to measure and aggregate anonymous user behavior data to improve our website.
Statistics These cookies measure - anonymously and aggregated - the success of our advertising. They measure visits and traffic sources in order to better tailor the website to your needs.
Marketing These cookies provide you with interest-based advertising. They measure the success of our advertising, anonymized and aggregated. These cookies are also used to deliver you personalized and interest based content.