Automated NGS library preparation on CyBio FeliX Application Note · Automated NGS Library Preparation on CyBio FeliX

Introduction

Automation of library preparation is very important to reduce opportunities for error, increase reproducibility, and reduce the amount of hands-on time allowing researchers to generate sequence data from DNA more efficiently.

By utilizing the CyBio® FeliX, a benchtop instrument for a variety of automated liquid handling tasks, the HaloPlex Target Enrichment System Kit for NGS library preparation for Ion Torrent™ Sequencing was automated to achieve reliable final prepared libraries with high accuracy for downstream sequencing. This application note describes the successful proof of principle to generate high-quality DNA libraries on CyBio® FeliX for personal genome analysis with the Ion Torrent™ Sequencing System.

Analytik Jena’s liquid handling portfolio includes CyBio® FeliX, a compact and flexible liquid handling platform for a variety of automated liquid handling tasks. The system has 12 deck positions on two levels for microplates, tube racks, reservoirs and tips on smallest footprint, including accessories like gripper, magnet adapter and thermal shaker.

Library preparation is a critical, hands-on and time-consuming step in the Next Generation Sequencing (NGS) workflow. To simplify the NGS library preparation process Analytik Jena developed a protocol for the HaloPlex Target Enrichment System Kit with minimal user intervention to prepare 8 library samples for multiplexed sequencing on the Ion Torrent™ platform.

The Institute for Laboratory Medicine in the Donauspital in Vienna was testing the CyBio® FeliX liquid handling platform to prepare DNA sample libraries from patient samples. Target enrichment of genomes greatly enhances the efficiency of Next Generation Sequencing, allowing researchers to focus on specific regions of interest. The Kit is suited to deep sequencing of small panels of genes (up to 2.5 Mb), such as those required for studies of inherited disorders, cancer, or infectious and neurologic diseases.