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Determination of Soil Microbial Biomass by TOC/TNb Measurement Application Note · multi N/C 2100S, multi N/C 3100

Challenge

  • Are the provided samples with high salt matrix (0.5 M of K2SO4 matrix) suitable for analysis on the multi N/C 2100S and multi N/C 3100?

Solution

  • Fast, safe and reproducible analysis with a high level of automation and very good long-term stability of the reactor filling.

Introduction

An important parameter in the site characterization of agricultural cultivation areas is the determination of microbial biomass in soils as well as dissolved organic matter (DOM), which are the basis of the diet of microorganisms.

For this purpose, the soil samples are extracted by aqueous salt solutions according to DIN EN ISO 14240-2 “Determination of soil microbial biomass, Part 2: Fumigation-extraction method”. The EOC (extractable organic carbon) or EN (extractable nitrogen) is determined by means of a NPOC/TN measurement.

For the determination of the microbial biomass, a second sample aliquot is fumigated according to the fumigation-extraction method with chloroform in order to lyse the microbial cells and thus to release the cell content, also making it available for the extraction. For this second step the extraction is conducted by means of salt solutions (typically used: 0.01 M CaCl2, 0.025–0.5 M K2SO4, 1 M KCl). From the difference calculation of the TOC/TN results of the fumigated and non-fumigated sample the soil content of microbial biomass can be determined.

The focus of this application is the instrumental TOC/TN analysis of the obtained soil extracts and their particular challenges to the analysis system.

 

Download the complete Application Note

Bestimmung der mikrobiellen Biomasse in Böden mittels TOC/TNb-Messung (DE)

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Determination of Soil Microbial Biomass by TOC/TNb Measurement (EN)

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